auto40 flow cytometer  (Apogee Flow Systems Ltd)

Journal: BMC Research Notes

Article Title: Single-platform, volumetric, CD45-assisted pan-leucogating flow cytometry for CD4 T lymphocytes monitoring of HIV infection according to the WHO recommendations for resource-constrained settings

doi: 10.1186/1756-0500-6-169

Figure Lengend Snippet: Gating strategy for volumetric, CD45-assisted panleucogating Auto40 flow cytometer (Apogee Flow Systems Ltd, Hemel Hempstead, UK), as depicted from typical figure provided by “Auto-Lymphocyte” software (Apogee Flow Systems Ltd) for the counting of CD4 T lymphocytes: A . CD45-PE-Dyomics649 x axis-light scatter dot plot scattergram; the primary CD45 gating differentiates clearly the CD45-positive lymphocytes and monocyte populations, and the population of polymorphonuclear cells; B . CD4-PE x axis-light scatter dot plot scattergram; double secondary CD4 gatings allow counting independently the population of CD4-negative lymphocyte and CD4-positive lymphocytes; C . Representative figure depicted by the software “Auto-Lymphocyte” in one blood sample taken as example: ( a ) CD45 (FL2)xside scatter (LS2) dot plot scattergram; ( b ) CD4 (FL1)xside scatter (LS2) dot plot scattergram; ( c ) Distribution of bright CD45 events during blood sample counting; ( d ) Distribution of bright CD4 events within the primary CD45 gating scattergram, during blood sample counting.

Article Snippet: The absolute and percentage CD4 T cell counts of 258 HIV-1-infected blood samples (182 adults and 76 children), living in N’Djamena, Chad, were performed by single-platform, volumetric, CD45-assisted pan-leucogating Auto40 flow cytometer (Apogee Flow Systems Ltd, Hemel Hempstead, UK) comparing to the FACSCalibur flow cytometer as a reference method.

Techniques: Flow Cytometry, Software

Journal: BMC Research Notes

Article Title: Single-platform, volumetric, CD45-assisted pan-leucogating flow cytometry for CD4 T lymphocytes monitoring of HIV infection according to the WHO recommendations for resource-constrained settings

doi: 10.1186/1756-0500-6-169

Figure Lengend Snippet: CD4 T cell counting in absolute cells/μl and percentage in 182 HIV-1-infected adults and 76 HIV-1-infected children older than 5 years, by the Apogee Auto40 flow cytometer at the hôpital Militaire D’Instruction, N’Djamena, Chad, and by the FACSCalibur at the hôpital de la Liberté, N’Djamena

Article Snippet: The absolute and percentage CD4 T cell counts of 258 HIV-1-infected blood samples (182 adults and 76 children), living in N’Djamena, Chad, were performed by single-platform, volumetric, CD45-assisted pan-leucogating Auto40 flow cytometer (Apogee Flow Systems Ltd, Hemel Hempstead, UK) comparing to the FACSCalibur flow cytometer as a reference method.

Techniques: Cell Counting, Flow Cytometry

Journal: BMC Research Notes

Article Title: Single-platform, volumetric, CD45-assisted pan-leucogating flow cytometry for CD4 T lymphocytes monitoring of HIV infection according to the WHO recommendations for resource-constrained settings

doi: 10.1186/1756-0500-6-169

Figure Lengend Snippet: Results from CD4 T cell count measurements in 182 HIV-1-infected adults and 76 HIV-1-infected children older than 5 years, expressed in absolute number (cells/μl) and in percentage carried out in parallel by the Auto40 flow cytometer and by the FACSCalibur. A , A ’, C and C ’. Passing-Bablok agreement tests between the CD4 T cell count results obtained by Auto40 and FACSCalibur flow cytometers, in absolute number ( A and A’ ) and in percentage ( C and C’ ). The diagonal dotted line represents the ideal line (no bias). The full line represents the regression line of the distribution; B , B ’, D and D ’. Bland-Altman analyses on the relative differences between the CD4 T cell counts obtained by Auto40 and FACSCalibur flow cytometers compared with the average CD4 T cell count, in absolute number ( B and B’ ) and in percentage ( D and D’ ). The full line represents the mean relative difference, and the dotted lines represent the superior and inferior limits of agreement. The arrow corresponds to the x abscise axis.

Article Snippet: The absolute and percentage CD4 T cell counts of 258 HIV-1-infected blood samples (182 adults and 76 children), living in N’Djamena, Chad, were performed by single-platform, volumetric, CD45-assisted pan-leucogating Auto40 flow cytometer (Apogee Flow Systems Ltd, Hemel Hempstead, UK) comparing to the FACSCalibur flow cytometer as a reference method.

Techniques: Cell Counting, Infection, Flow Cytometry

Journal: BMC Research Notes

Article Title: Single-platform, volumetric, CD45-assisted pan-leucogating flow cytometry for CD4 T lymphocytes monitoring of HIV infection according to the WHO recommendations for resource-constrained settings

doi: 10.1186/1756-0500-6-169

Figure Lengend Snippet: The sensitivity and specificity of CD4 T cell counting by the Auto40 to identify patients having less than (or more than) 200 CD4 T cells/μl, 350 CD4 T cells/μl, 750 CD4 T cells/μl and 25%CD4+, calculated on the 258 available CD4 T cell count measurements

Article Snippet: The absolute and percentage CD4 T cell counts of 258 HIV-1-infected blood samples (182 adults and 76 children), living in N’Djamena, Chad, were performed by single-platform, volumetric, CD45-assisted pan-leucogating Auto40 flow cytometer (Apogee Flow Systems Ltd, Hemel Hempstead, UK) comparing to the FACSCalibur flow cytometer as a reference method.

Techniques: Cell Counting

Journal: BMC Research Notes

Article Title: Single-platform, volumetric, CD45-assisted pan-leucogating flow cytometry for CD4 T lymphocytes monitoring of HIV infection according to the WHO recommendations for resource-constrained settings

doi: 10.1186/1756-0500-6-169

Figure Lengend Snippet: CD4 T cell counting in absolute count and percentage in 258 HIV-1-infected individuals, by the Apogee Auto40 flow cytometer at the hôpital Militaire D’Instruction, N’Djamena, Chad, and by the FACSCalibur at the hôpital de la Liberté, N’Djamena, at various CD4 T cell count ranges according to the FACSCalibur results

Article Snippet: The absolute and percentage CD4 T cell counts of 258 HIV-1-infected blood samples (182 adults and 76 children), living in N’Djamena, Chad, were performed by single-platform, volumetric, CD45-assisted pan-leucogating Auto40 flow cytometer (Apogee Flow Systems Ltd, Hemel Hempstead, UK) comparing to the FACSCalibur flow cytometer as a reference method.

Techniques: Cell Counting, Flow Cytometry

Journal: BMC Research Notes

Article Title: Single-platform, volumetric, CD45-assisted pan-leucogating flow cytometry for CD4 T lymphocytes monitoring of HIV infection according to the WHO recommendations for resource-constrained settings

doi: 10.1186/1756-0500-6-169

Figure Lengend Snippet: Gating strategy for volumetric, CD45-assisted panleucogating Auto40 flow cytometer (Apogee Flow Systems Ltd, Hemel Hempstead, UK), as depicted from typical figure provided by “Auto-Lymphocyte” software (Apogee Flow Systems Ltd) for the counting of CD4 T lymphocytes: A . CD45-PE-Dyomics649 x axis-light scatter dot plot scattergram; the primary CD45 gating differentiates clearly the CD45-positive lymphocytes and monocyte populations, and the population of polymorphonuclear cells; B . CD4-PE x axis-light scatter dot plot scattergram; double secondary CD4 gatings allow counting independently the population of CD4-negative lymphocyte and CD4-positive lymphocytes; C . Representative figure depicted by the software “Auto-Lymphocyte” in one blood sample taken as example: ( a ) CD45 (FL2)xside scatter (LS2) dot plot scattergram; ( b ) CD4 (FL1)xside scatter (LS2) dot plot scattergram; ( c ) Distribution of bright CD45 events during blood sample counting; ( d ) Distribution of bright CD4 events within the primary CD45 gating scattergram, during blood sample counting.

Article Snippet: Direct volumetric CD4 T cell measurements were performed on the Auto40 using phycoerythrin (PE)-conjugated anti-CD4 and PE-Dyomics649-conjugated anti-CD45 monoclonal antibodies (Apogee Flow Systems Ltd).

Techniques: Flow Cytometry, Software

Journal: BMC Research Notes

Article Title: Single-platform, volumetric, CD45-assisted pan-leucogating flow cytometry for CD4 T lymphocytes monitoring of HIV infection according to the WHO recommendations for resource-constrained settings

doi: 10.1186/1756-0500-6-169

Figure Lengend Snippet: CD4 T cell counting in absolute cells/μl and percentage in 182 HIV-1-infected adults and 76 HIV-1-infected children older than 5 years, by the Apogee Auto40 flow cytometer at the hôpital Militaire D’Instruction, N’Djamena, Chad, and by the FACSCalibur at the hôpital de la Liberté, N’Djamena

Article Snippet: Direct volumetric CD4 T cell measurements were performed on the Auto40 using phycoerythrin (PE)-conjugated anti-CD4 and PE-Dyomics649-conjugated anti-CD45 monoclonal antibodies (Apogee Flow Systems Ltd).

Techniques: Cell Counting, Flow Cytometry

Journal: BMC Research Notes

Article Title: Single-platform, volumetric, CD45-assisted pan-leucogating flow cytometry for CD4 T lymphocytes monitoring of HIV infection according to the WHO recommendations for resource-constrained settings

doi: 10.1186/1756-0500-6-169

Figure Lengend Snippet: Results from CD4 T cell count measurements in 182 HIV-1-infected adults and 76 HIV-1-infected children older than 5 years, expressed in absolute number (cells/μl) and in percentage carried out in parallel by the Auto40 flow cytometer and by the FACSCalibur. A , A ’, C and C ’. Passing-Bablok agreement tests between the CD4 T cell count results obtained by Auto40 and FACSCalibur flow cytometers, in absolute number ( A and A’ ) and in percentage ( C and C’ ). The diagonal dotted line represents the ideal line (no bias). The full line represents the regression line of the distribution; B , B ’, D and D ’. Bland-Altman analyses on the relative differences between the CD4 T cell counts obtained by Auto40 and FACSCalibur flow cytometers compared with the average CD4 T cell count, in absolute number ( B and B’ ) and in percentage ( D and D’ ). The full line represents the mean relative difference, and the dotted lines represent the superior and inferior limits of agreement. The arrow corresponds to the x abscise axis.

Article Snippet: Direct volumetric CD4 T cell measurements were performed on the Auto40 using phycoerythrin (PE)-conjugated anti-CD4 and PE-Dyomics649-conjugated anti-CD45 monoclonal antibodies (Apogee Flow Systems Ltd).

Techniques: Cell Counting, Infection, Flow Cytometry

Journal: BMC Research Notes

Article Title: Single-platform, volumetric, CD45-assisted pan-leucogating flow cytometry for CD4 T lymphocytes monitoring of HIV infection according to the WHO recommendations for resource-constrained settings

doi: 10.1186/1756-0500-6-169

Figure Lengend Snippet: The sensitivity and specificity of CD4 T cell counting by the Auto40 to identify patients having less than (or more than) 200 CD4 T cells/μl, 350 CD4 T cells/μl, 750 CD4 T cells/μl and 25%CD4+, calculated on the 258 available CD4 T cell count measurements

Article Snippet: Direct volumetric CD4 T cell measurements were performed on the Auto40 using phycoerythrin (PE)-conjugated anti-CD4 and PE-Dyomics649-conjugated anti-CD45 monoclonal antibodies (Apogee Flow Systems Ltd).

Techniques: Cell Counting

Journal: BMC Research Notes

Article Title: Single-platform, volumetric, CD45-assisted pan-leucogating flow cytometry for CD4 T lymphocytes monitoring of HIV infection according to the WHO recommendations for resource-constrained settings

doi: 10.1186/1756-0500-6-169

Figure Lengend Snippet: CD4 T cell counting in absolute count and percentage in 258 HIV-1-infected individuals, by the Apogee Auto40 flow cytometer at the hôpital Militaire D’Instruction, N’Djamena, Chad, and by the FACSCalibur at the hôpital de la Liberté, N’Djamena, at various CD4 T cell count ranges according to the FACSCalibur results

Article Snippet: Direct volumetric CD4 T cell measurements were performed on the Auto40 using phycoerythrin (PE)-conjugated anti-CD4 and PE-Dyomics649-conjugated anti-CD45 monoclonal antibodies (Apogee Flow Systems Ltd).

Techniques: Cell Counting, Flow Cytometry